The Polizzi lab is seeking a Research Technician to contribute to experimental pipelines for the characterization of designed small-molecule binding proteins. This entry-level position is a great opportunity to gain experience working in a cutting-edge protein design laboratory.??
The Lab uses a mix of computational and experimental approaches to design and characterize ligand-binding proteins. We develop structural bioinformatics and machine-learning techniques to compute the structures and sequences of proteins that bind to challenging small-molecule ligands. We then express these proteins in the lab and characterize them with biophysical and structural methods (see, e.g., Polizzi and DeGrado, Science 2020).
We aim not only to unlock the design principles used by proteins for molecular recognition but also to make useful proteins for probing and manipulating biology. By designing de novo small-molecule-binding proteins, we can isolate the essential ingredients for protein molecular recognition events, obtain atomic-level control of the protein/small-molecule interface, and set the stage for the development of proteins that could serve as novel sensors, enzymes, drug delivery vehicles, or pharmaceuticals.
We are seeking enthusiastic individuals who possess strong interpersonal skills, have a growth mindset, are highly organized, and who interact well with others as part of a collaborative team.
The ideal candidate will have laboratory experience in protein biochemistry (protein expression and purification using E. coli, yeast, or mammalian cell systems, as well as basic molecular biology techniques) or a willingness to learn. Some experience in structural methods (X-ray crystallography, electron microscopy, or NMR) and/or protein-display methods (e.g., yeast-, phage-, or ribosome-display) would be highly desirable.?? While this role is predominantly focused on performing wet-lab experiments, there is also ample opportunity for the candidate to learn some computational protein design skills while on the job.
Candidates with a background that encompasses both dry- and wet-lab experience are also highly encouraged to apply.
Our lab at Dana-Farber???s Longwood Center has access to state-of-the-art facilities, including for cryo-EM, X-ray crystallography, flow cytometry, and high-performance computing. We are part of a highly collaborative, interdisciplinary environment within the Chemical Biology program at the Dana-Farber Cancer Institute and the Department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School.?? We collaborate with leading biologists and physician scientists to pursue high impact applications of protein design for understanding and controlling biology.??
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS and related diseases.??
The candidate is expected to attend team meetings to share results, as well as document findings in oral and written reports.
We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong.?? As relentless as we are in our mission to reduce the burden of cancer for all, we are equally committed to diversifying our faculty and staff.?? Cancer knows no boundaries and when it comes to hiring the most dedicated and diverse professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.
Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law.
About Dana-Farber Cancer Institute
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to under-served members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
Connections working at Dana-Farber Cancer Institute...faculty, staff, and guests of the college. Other duties as assigned. Education and/or Experience A minimum of an associate degree or higher in criminal justice or a related field, one to three years of related work experience or a combination of education...
...Job Description We're Hiring an Elementary School Secretary! Are you a detail-oriented, organized individual with a passion for education? St. Francis Indian School AKA Sicangu Oyate Ho Inc. is seeking a dedicated Elementary School Secretary to join our team in St....
...Job Description and Duties * This advertisement is for a Jewish Chaplain positionlocated at Pelican Bay State Prison in Crescent City, Del Norte County. Please indicate the position number on your application: (***) ***-****-001. ** Note: Main communication for this...
...to join our quest for better? Job Description The Sonographer reflects the mission, vision, and values of NM, adheres to... ...Qualifications Required Graduate of an accredited Ultrasound Sonography program Registered Diagnostic Medical Sonographer...
...transforming lives for over 40 years! As a Christian non-profit, Prison Fellowship trains and... ...solutions in SaaS systems ~ Experience working with engineering teams ~ Practical... ...communication skills ~ This is a remote/work from home position in the US with a 5% travel...