Laboratory Technician | Johns Hopkins University Job at Johns Hopkins University, Saint Petersburg, FL

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  • Johns Hopkins University
  • Saint Petersburg, FL

Job Description

The Nagy Laboratory at the Johns Hopkins School of Medicine/Johns Hopkins All Children's Hospital is seeking to hire a Laboratory Technician . The Laboratory Technician primary focus in the Nagy laboratory is to study epigenomics of cell type specification and the role of macrophages in tissue regeneration, metabolism and tumor growth and metastasis. The individual in this role will assist in experiments in these research areas and help with general lab needs. The Laboratory Technician is expected to have some knowledge of cell culture and basic molecular biology techniques (PCR, Western blot, molecular cloning). This position will report directly to the lab manager for day-to-day operations.


Specific Duties & Responsibilities: 

  • Performs routine lab duties such as preparing buffers and stocking lab supplies.
  • Collect data and enter/organize mouse information into Excel spreadsheets.
  • Perform genotyping on tissue samples.
  • Perform qPCR or western blot experiments on cell or tissue samples.
  • Carry out in vitro cell culture.
  • Assist other lab members in preparing or carrying out similar experiments.
  • Run lab tests and conduct measurements under established protocols and under supervision.
  • Report problems in completion of routine laboratory processes.


Knowledge, Skills, & Abilities:


  • Molecular biology techniques such as DNA and RNA isolation, PCR, agarose gel electrophoresis, qPCR, and western blotting.


Physical Requirements:

  • Employee may remain in a stationary position (sitting or standing) for up to 40% of the time.
  • Employee is required to operate small laboratory tools that require manual dexterity.
  • Prolonged vision requirements include viewing computer screen, paper reports/documents, charts and results.
  • Visual acuity is required to distinguish fine gradation of color or structure and closely examine specimens (including via a microscope).
  • May transport equipment and supplies usually less than 40 pounds from one area to another (in the laboratory).
  • May be required to lift and/or move up to 50 pounds with proper training, or precautions/lifting aides (example: supply boxes).
  • In some areas workspace is limited.
  • Hours of work may vary from week to week depending on experiment needs.


Laboratory Environment

  • Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed.
  • Must refer to and are expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas.
  • Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area.
  • Working in laboratory where there may be discomforts due to odors, noise, temperature fluctuations, and working around lab equipment.
  • Research areas may include animal, human, human products, DNA, and radiation protocols.
  • Use of personal protective equipment may be advised or required.






Minimum Qualifications

  • High school diploma/GED required.
  • Some coursework in the fields of biology, chemistry, or molecular biology with some prior laboratory experience.




Preferred Qualifications

  • College coursework in biology, chemistry or related field.
  • At least 6 months experience working in a biomedical research laboratory.
  • Certificate from technical institute or internship experience.

Classified Title: Laboratory Technician 
Role/Level/Range: ACRO40/E/02/CB  
Starting Salary Range: $15.25 - $23.00 HRLY (Commensurate with experience) 
Employee group: Full Time 
Schedule: M – F (8:30 am – 5 pm) 
Exempt Status: Non-Exempt 
Location: Florida 
Department name: ​​​​​​​All Childrens 
Personnel area: School of Medicine 

About Johns Hopkins University

Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal ... timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.

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Job Tags

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